The “Universal Exhibition of Seville” was held in Seville, Spain, from April 20 to October 12, 1992. Organized by Expo '92, a state company, it was a universal exhibition under the Second Categorization System, and it commemorated the 500th anniversary of Christopher Columbus's arrival in the Americas. Over 176 days the Expo recorded 41,814,571 visits, according to the BIE. That figure was more than twice what the organizers had expected and larger than the population of Spain at the time.

Spain proposed the Expo to present the country's new position in the world after the death of Francisco Franco, and King Juan Carlos I announced the bid shortly after he came to the throne. The BIE's 93rd General Assembly registered the Expo on June 15, 1983. The quincentenary was at first meant to be marked on both sides of the Atlantic, with a twin exhibition in Chicago, the proposed Age of Discovery International Exposition. Planning in Chicago ended in 1985 because of escalating costs, and Seville went ahead alone. Spain saw the event as a chance to display its cultural diversity, including pavilions for its autonomous regions, and to strengthen its standing in Europe and beyond.

The theme was “The Age of Discovery.” The Expo was built on Isla de la Cartuja, an island in the Guadalquivir River linked to Columbus's preparations for his voyage. Its only existing structure was the La Cartuja monastery, where Columbus had stayed. The monastery had later served as a ceramics factory and fallen into disrepair, and it was restored for the Expo. The main avenue, the Road of the Discoveries, was lined with 10 thematic pavilions that traced 500 years of science, technology and culture. Along the way visitors could board a 15th-century caravel and then a spaceship.

The Expo also gave real attention to conservation. A Pavilion of the Environment and an Energy Pavilion addressed resources, and the Pavilion of Nature included a scale model of the Amazon rainforest to explain deforestation. The United Kingdom pavilion, designed by Grimshaw, used modern techniques to stay cool with minimal energy.

The Expo was planned during the Cold War but opened after it had ended, and world events reshaped the list of participants. German reunification meant that a single German pavilion, by Georg Lippsmeier, replaced the two pavilions once planned. The Soviet pavilion became the Russian Pavilion, and Estonia, Latvia and Lithuania shared a Baltic pavilion. The Gulf War kept Iraq from taking part. In all, the BIE counts 108 participants. The European Community had its own pavilion on the Avenue of Europe. The United States pavilion was scaled back for budget reasons: most of Barton Myers's original design was dropped in favor of two geodesic domes that had previously been used in the Soviet Union.

Evenings were a large part of the Expo's appeal. Nightly fireworks and light shows over the Lake of Spain, known as “Expo-night,” were joined by opera, orchestras, theater, jazz, flamenco and salsa, with some performances running until 4 a.m. The mascot, Curro, was chosen from 23 designs submitted by entrants from 15 countries. Its name comes from an Andalusian nickname for men named Francisco. Curro is a mostly white bird with elephant-like legs and a multicolored crest and beak, with each of five colors representing a continent.

The Expo transformed the region's infrastructure. Seville gained a new airport terminal, a single train station that replaced two older ones, a high-speed rail line to Madrid, new highways and four bridges: Barqueta, Alamillo, Cartuja and El Cachorro. The project also raised the profile of Andalusia by demonstrating its organizational and logistical capabilities.

After the Expo closed, Cartuja Island became Cartuja 93, a center for technology and economic development now known as the Cartuja Science and Technology Park. Many pavilions found new uses. The Moroccan pavilion houses the Three Cultures of the Mediterranean Foundation, the French pavilion has hosted a start-up incubator, the Italian pavilion holds offices for technology companies, and the Puerto Rican pavilion was converted into offices. Sweden's wooden pavilion was moved home and now houses the Grythyttan Academy for Culinary Arts, while the Aragon pavilion became the headquarters of a regional employers' federation in Zaragoza. The restored monastery is now the Andalusian Center for Contemporary Art, the General Archive of Andalusia has its headquarters on the site, and an amusement park was built near the Lake of Spain.

Seville continues to mark the anniversaries. In 2017, King Juan Carlos attended the ceremony that opened a program of more than 30 activities for the 25th anniversary, and guided tours visited 32 surviving pavilions. The citizen association Legado Expo Sevilla leads tours of the former grounds, and the 30th anniversary in 2022 brought exhibitions and concerts back to the site. Expo 1992 Seville gave a newly democratic Spain a global stage and left Seville with infrastructure and a technology district that still shape the city.